Welcome to THC Testing using Cannabis Test Kits (CTK)™


This site explains Thin Layer Chromatography (TLC) testing cannabis plant material and products made from plant material. This test will identify various cannabinoids which includes THC, CBC, CBG, and many other compounds. THC testing can be economically and financially beneficial for cannabis growers, individuals, producers, vendors, and marijuana retail markets. Personal THC testing is a novel approach that has been gaining interest in the marijuana arena. The testing process is not complicated or time consuming, but there are some techniques and tricks that a THC class with the right lab equipment will facilitate in simplifying the process.


What is TLC Testing?

TLC stands for Thin Layer Chromatography.  It is one of the various methods used to visualize the chemical compounds within a molecular structure.


How does this apply to Cannabis?


Cannabis Testing Kit™ was developed to specifically identify cannabis compounds.  The kit has all the components necessary to run the TLC tests and tools to interpret the test results


How does CTK™ work?

TLC solvent

Cannabis Testing Kit™ uses the concept of capillary action and Rf values. Special reagents are used for extraction of the cannabis and operation of the TLC plates. Once the cannabis is extracted, the cannabinoid sample is placed on a small glass plate impregnated with material that will separate out the individual compounds. Capillary action moves the compounds up the TLC plate.  When enough time has passed, the cannabinoids, along with other cannabis components, will be separated out.  

*Dots seen on the diagram is for illustration purposes only. There is no actual visualization of the sample moving up the plate.


Final Visual Plate

If the TLC test is done correctly, there will be separation of the different cannabinoid components. A specific dye is sprayed onto the TLC plate that will colorize the samples. You can then visualize what kind of chemotype profile the sample contains and how much cannabinoid products are present. The distance a compound has moved up the plate is measured against the total migration of the solvent, this measurement is called an Rf value. Rf stands for Retention factor. Retention factor values have been been determined for different cannabinoids and displayed on the provided cannabis fingerprinting chart.


Cannabinoid Interpretation

CBN fingerprint

Cannabinoid profiles that determine the chemotypes of the cannabis sample are analyzed using the fingerprinting chart (see diagram). Potency of the sample are estimated using a special measuring tool that checks the amount of THC. A more detailed explanation is in the manual which you will receive once CTK™ is purchased or sign up for the THC testing class.


CTK™ Classes

confusedIf this explanation is still confusing we offer CTK™ classes to get hands on training for confident performance and understanding of this test.  At the end of each class, students that have shown to be proficient will receive a CTK™ competency certificate.


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